个人简介

陈文沅，物理学博士。2020年12月博士毕业于新·葡萄88805。2020年12月，进入新·葡萄88805生物学科研流动站工作。2022年12月，任职于新·葡萄88805。主要研究方向为冷冻电镜噬菌体非对称重构方法学及其应用。在Science，Nature Communications，PNAS，Protein and Cell等期刊上发表研究论文10余篇。

Email:wenyuanchen@hunnu.edu.cn

研究方向

软物质与生物物理；冷冻电镜噬菌体病毒三维重构算法与结构解析

教学情况

本科课程：大学物理冷冻电镜病毒三维重构虚拟仿真实验

承担课题

1.国家自然科学基金青年基金，32200994，冷冻电镜短尾噬菌体T7核心蛋白结构解析及基因组释放机制研究，2023/01-2025/12，主持

2.国家自然科学基金重点项目，12034006，冷冻电镜病毒非对称重构研究，2021/01-2025-12，参与

3.国家自然科学基金面上项目，32071209，冷冻电镜噬菌体对称失配重构研究，2021/01-2024/12，课题骨干

4.中国博士后科学基金第3批特别资助（站前），2021TQ0104，冷冻电镜噬菌体对称失配近原子分辨三维重构研究，2021/06-2022/11，主持

5.中国博士后科学基金第71批面上资助二等，2022M711127，冷冻电镜噬菌体T7衣壳蛋白原子级分辨率三维重构研究，2022/06-2022/11，主持

6.国家重点研发计划，2016YFA0501103，冷冻电镜原子分辨结构解析高效算法的开发与应用，2016/07-2021/06，参与

代表性论文

1. Xiao Hao, Zhou Junquan, Yang Fan, Liu Zheng, Song Jingdong,Chen Wenyuan*, Liu Hongrong*, Cheng Lingpeng*. Assembly and Capsid Expansion Mechanism of Bacteriophage P22 Revealed by High-Resolution Cryo-EM Structures.Viruses. 2023; 15(2):355.

2. Jing Zheng,Wenyuan Chen, Hao Xiao, Fan Yang, Xiaowu Li, Jingdong Song and Lingpeng Cheng*, Hongrong Liu*. A capsid structure ofRalstonia solanacearum podoviridaeGP4 witha triangulation number T = 9. Viruses. 2022; 14(11):2431.

3. Chen Wenyuan, Xiao Hao, Wang Li, Wang Xurong, Tan Zhixue, Han Zhen, Li Xiaowu, Yang Fan, Liu Zhonghua, Song Jingdong*, Liu Hongrong*, Cheng Lingpeng*. Structural changes in bacteriophage T7 upon receptor-induced genome ejection. Proceedings of the National Academy of Sciences of the United States of America. 2021; 118 (37): e2102003118.

4. Wenyuan Chen, Hao Xiao, Xurong Wang, Shuanglin Song, Zhen Han, Xiaowu Li, Fan Yang, Li Wang, Jingdong Song*, Hongrong Liu*, Lingpeng Cheng*，Structural changes of a bacteriophage upon DNA packaging and maturation,Protein & Cell. 11(2020) pp. 374–379.

5. Nan Wang,Wenyuan Chen, Ling Zhu, Dongjie Zhu, Rui Feng, Jialing Wang, Bin Zhu, Xinzheng Zhang, Xiaoqing Chen, Xianjie Liu, Runbin Yan, Dongyao Ni, Grace Guoying Zhou, Hongrong Liu*, Zihe Rao*, Xiangxi Wang*, Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation,Protein & Cell. 11(2020) pp. 366–373.

6. Xiaowu Li, Li Wang, Xurong Wang,Wenyuan Chen, Tao Yang, Jingdong Song, Hongrong Liu, Lingpeng Cheng, Structure of RdRps within a transcribing dsDNA virus provides insights into the mechanisms of RNA synthesis, Journal of Molecular Biology. 432(2020) pp. 358-366.

7. Jialing Wang, Shuai Yuan, Dongjie Zhu, Hao Tang, Nan Wang,Wenyuan Chen, Qiang Gao, Yuhua Li, Junzhi Wang, Hongrong Liu*, Zihe Rao*, Xiangxi Wang*, Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component,Nature Communications. 9(2018) DOI: 10.1038/s41467-018-06078-4.

8. Shuai Yuan, Jialing Wang, Dongjie Zhu, Nan Wang, Qiang Gao,Wenyuan Chen, Hao Tang, Junzhi Wang, Xinzheng Zhang*, Hongrong Liu*, Zihe Rao*, Xiangxi Wang*, Cryo-EM structure of a herpesvirus capsid at 3.1 angstrom,Science. 360(2018) pp. 48-+.

9. Xurong Wang, Fuxian Zhang, Rui Su, Xiaowu Li,Wenyuan Chen, Qingxiu Chen, Tao Yang, Jiawei Wang, Hongrong Liu*, Qin Fang*, Lingpeng Cheng*, Structure of RNA polymerase complex and genome within a dsRNA virus provides insights into the mechanisms of transcription and assembly,Proceedings of the National Academy of Science, USA. 115(2018): 7344-7349.

10. Xiaowu Li, Niyun Zhou,Wenyuan Chen, Bin Zhu, Xurong Wang, Bin Xu, Jiawei Wang, Hongrong Liu*, Lingpeng Cheng*, Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200 kV,Journal of Molecular Biology. 429(2017) pp. 79-87.